dba 2jrj mice  (Janvier Labs)

Journal: Applied Microbiology and Biotechnology

Article Title: OP7, a novel influenza A virus defective interfering particle: production, purification, and animal experiments demonstrating antiviral potential

doi: 10.1007/s00253-020-11029-5

Figure Lengend Snippet: Mouse infection experiments with OP7 material. Female 8–12-week-old D2- Mx1 r/r mice were intra-nasally infected with 20 μL solution containing OP7 and/or STV in PBS. a , b Toxicity was tested by applying 10 μL active ( n = 5) or inactive ( n = 5) OP7 material individually (dose: 2.2E+8 Seg7 OP7 vRNA copies/mice). All mice survived the treatment with active or inactive OP7. c , d Mice were co-treated with 10 μL active ( n = 9) or inactive ( n = 13) OP7 material (dose: 2.2E+8 Seg7 OP7 vRNA copies/mice) and a lethal dose of 1000 FFU STV. e , f Mice were infected with a lethal dose of 1000 FFU STV and treated with different dilutions of active OP7 (1:2, dose: 2.2E+8 Seg7 OP7 vRNA copies/mice ( n = 4); 1:10, dose: 4.4E+7 Seg7 OP7 vRNA copies/mice ( n = 6); 1:20, dose: 2.2E+7 Seg7 OP7 vRNA copies/mice ( n = 4); 1:50, dose: 8.8E+6 Seg7 OP7 vRNA copies/mice ( n = 6)) or PBS ( n = 10). All mice treated with active OP7 diluted 1:2 or 1:10 survived the infection. Y -axes show percentages (in relation to day 0) of mean body weight losses ( a , c , e ) and survival rates ( b , d , e ) for each group. Error bars indicate one standard error of the mean (SEM) for body weight changes

Article Snippet: DBA/2JRj (D2- Mx1 −/− ) mice carrying a non-functional Mx1 gene were obtained from Janvier.

Techniques: Infection